Anticorps Recombinant de lapin anti-Phospho-STAT5A (Tyr694)

Phospho-STAT5A (Tyr694) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2P21

N° de cat : CL647-80115

Synonymes

MGF, Phospho-STAT5A (Tyr694), STAT5, STAT5A

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Galerie de données de validation

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  FC experiment of TF-1 using CL647-80115

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.06 ug CoraLite® Plus 647 Anti-Human Phospho-STAT5A (Tyr694) (CL647-80115, Clone:2P21), or 0.06 ug Control Antibody (blue, solid line). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  FC experiment of TF-1 using CL647-80115

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.06 ug CoraLite® Plus 647 Anti-Human Phospho-STAT5A (Tyr694) (CL647-80115, Clone:2P21), or 0.06 ug Control Antibody (blue, solid line). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  FC experiment of TF-1 using CL647-80115

  1X10^6 TF-1 cells untreated (dashed line, low) or treated with GM-CSF (100 ng/mL at 37℃ for 30 minutes after serum- starvation overnight, red, positive) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-STAT5A (Tyr694) (CL647-80115, Clone:2P21) (red), or 0.13 ug Control Antibody (blue, solid line). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en cytométrie: cellules TF-1, cellules TF-1 traitées au GM-CSF

Dilution recommandée

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-80115 cible Phospho-STAT5A (Tyr694) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 5A
• Masse moléculaire calculée: 794 aa, 92 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: BC027036
• Symbole du gène: STAT5A
• Identification du gène (NCBI): 6776
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.