Anticorps Polyclonal de lapin anti-Phospho-STAT5A (Tyr694)/STAT5B (Tyr699)

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 28949-1-AP

Synonymes

MGF, Phospho-STAT5A (Tyr694), STAT5, STAT5A

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Galerie de données de validation

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  WB analysis using 28949-1-AP

  Non-treated TF-1 and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 28949-1-AP (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules TF-1 traitées au GM-CSF,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28949-1-AP cible Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 5A
• Masse moléculaire calculée: 794 aa, 92 kDa
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: BC027036
• Symbole du gène: STAT5A
• Identification du gène (NCBI): 6776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.