Anticorps Monoclonal anti-Phospho-RPS6KA1 (Thr359/Ser363)

Phospho-RPS6KA1 (Thr359/Ser363) Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2C11D9

N° de cat : 68041-1-Ig

Synonymes

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

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Galerie de données de validation

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  WB analysis using 68041-1-Ig

  Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control.

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  WB analysis using 68041-1-Ig

  Non-treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control.

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  IF Staining of HeLa using 68041-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules HeLa traitées à la λ phosphatase, cellules NIH/3T3 traitées à la λ phosphatase
• Résultats positifs en IF: cellules HeLa traitées à la λ phosphatase,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68041-1-Ig cible Phospho-RPS6KA1 (Thr359/Ser363) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Masse moléculaire calculée: 735 aa, 83 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC014966
• Symbole du gène: RPS6KA1
• Identification du gène (NCBI): 6195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)