Anticorps Polyclonal de lapin anti-Phospho-RPS6KA1 (Ser380)

Phospho-RPS6KA1 (Ser380) Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 28983-1-AP

Synonymes

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Ser380), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 28983-1-AP

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28983-1-AP (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

---

Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28983-1-AP cible Phospho-RPS6KA1 (Ser380) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Masse moléculaire calculée: 735 aa, 83 kDa
• Poids moléculaire observé: 83-90 kDa
• Numéro d’acquisition GenBank: BC014966
• Symbole du gène: RPS6KA1
• Identification du gène (NCBI): 6195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer; Authors - Jing Chen
human	WB	Cell Death Dis; Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration; Authors - Yunfeng Hu