Anticorps Monoclonal anti-Phospho-RIPK1 (Ser161)

Phospho-RIPK1 (Ser161) Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B2G1

N° de cat : CL647-66854

Synonymes

Cell death protein RIP, FLJ39204, Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

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Galerie de données de validation

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  IF Staining of U2OS using CL647-66854

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 647 Phospho-RIPK1 (Ser161) antibody (CL647-66854, Clone: 1B2G1 ) at dilution of 1:400.

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  FC experiment of HEK-293T using CL647-66854

  1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RIPK1 (Ser161) (CL647-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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  FC experiment of HEK-293T using CL647-66854

  1X10^6 HEK-293T cells were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RIPK1 (Ser161) (CL647-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en IF: cellules U2OS,
• Résultats positifs en cytométrie: cellules HEK-293T traitées à la calyculine A, cellules HEK-293T

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-66854 cible Phospho-RIPK1 (Ser161) dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 74 kDa, 45 kDa
• Numéro d’acquisition GenBank: NM_003804
• Symbole du gène: RIPK1
• Identification du gène (NCBI): 8737
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).