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Anticorps Monoclonal anti-Phospho-RIPK1 (Ser161)

Phospho-RIPK1 (Ser161) Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1B2G1

N° de cat : CL594-66854

Synonymes

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161) , Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite®594-conjugated Phospho-RIPK1 (Ser161) Mo (CL594-66854)

    FC experiment of HEK-293T using CL594-66854

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®594 Anti-Human Phospho-RIPK1 (Ser161) (CL594-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite®594-conjugated Phospho-RIPK1 (Ser161) Mo (CL594-66854)

    FC experiment of PC-3 using CL594-66854

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.06 ug CoraLite®594 Anti-Human Phospho-RIPK1 (Ser161) (CL594-66854, Clone:1B2G1) (red), or 0.06 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Applications testées

Résultats positifs en cytométriecellules HEK-293T traitées à la calyculine A, cellules PC-3 traitées à la calyculine A

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66854 cible Phospho-RIPK1 (Ser161) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet receptor (TNFRSF)-interacting serine-threonine kinase 1
Masse moléculaire calculée 76 kDa
Poids moléculaire observé 74 kDa, 45 kDa
Numéro d’acquisition GenBankNM_003804
Symbole du gène RIPK1
Identification du gène (NCBI) 8737
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25 kDa and 45 kDa in HAEC and HUVEC (PMID: 22685397).