Anticorps Polyclonal de lapin anti-Phospho-PRKD1 (Ser916)

Phospho-PRKD1 (Ser916) Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 28928-1-AP

Synonymes

Phospho-PRKD1 (Ser916), PKC MU, PKCM, PKD, PKD1, PRKCM, PRKD1, Protein kinase C mu type, Protein kinase D, protein kinase D1

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Galerie de données de validation

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  WB analysis using 28928-1-AP

  Non-treated NIH/3T3 and Insulin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28928-1-AP (Phospho-PRKD1 (Ser916) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées à l'insuline,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28928-1-AP cible Phospho-PRKD1 (Ser916) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase D1
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: NM_001330069
• Symbole du gène: PRKD1
• Identification du gène (NCBI): 5587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3, et BSA à 0,05 %.
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)

Publications

Species	Application	Title
rat	WB	Food Chem Toxicol; G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.; Authors - Yao Liu
rat	WB	Biochem Biophys Res Commun; Protein kinase D1 promotes the survival of random-pattern skin flaps in rats; Authors - Jianpeng Chen