Anticorps Polyclonal de lapin anti-Phospho-PERK/EIF2AK3 (Ser719)

Phospho-PERK/EIF2AK3 (Ser719) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 29546-1-AP

Synonymes

DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, Phospho-PERK/EIF2AK3 (Ser719), WRS

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Galerie de données de validation

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  WB analysis using 29546-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29546-1-AP

  Non-treated HeLa cells and Thapsigargin treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la λ phosphatase, Thapsigargin treated HeLa cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

29546-1-AP cible Phospho-PERK/EIF2AK3 (Ser719) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 3
• Masse moléculaire calculée: 1116 aa, 125 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC126354
• Symbole du gène: EIF2AK3
• Identification du gène (NCBI): 9451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Publications

Species	Application	Title
human	WB	Mar Drugs; Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma; Authors - Yun Huang
human	WB	World J Gastroenterol; Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress; Authors - Xiao-Xue Hou
human	WB	Tissue Cell; XBP1s gene of endoplasmic reticulum stress enhances proliferation and osteogenesis of human periodontal ligament cells; Authors - Ziwei Cui
	WB	Fish Shellfish Immunol; Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp; Authors - Jie Cui
human	WB	Cell Death Dis; PDIA4 confers resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell carcinoma; Authors - Lichun Kang
rat	WB	Ecotoxicol Environ Saf; The ZIP9-centered androgen pathway compensates for the 2605 MHz radiofrequency electromagnetic radiation-mediated reduction in resistance to H2O2 damage in Sertoli cells of adult rats; Authors - Gang Yu