Anticorps Polyclonal de lapin anti-Phospho-PDH E1 Alpha (Ser232)

Phospho-PDH E1 Alpha (Ser232) Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 29582-1-AP

Synonymes

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A, Phospho-PDH E1 Alpha (Ser232)

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Galerie de données de validation

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  WB analysis using 29582-1-AP

  10 % fetal bovine serum treated HeLa cells and Serum-free treated HeLa cells were subjected to SDS PAGE followed by western blot with 29582-1-AP (Phospho-PDH E1 Alpha (Ser232) antibody) at dilution of 1:4000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of HepG2 using 29582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser232) antibody (29582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, Serum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells
• Résultats positifs en IF: λ phosphatase treated HepG2 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

29582-1-AP cible Phospho-PDH E1 Alpha (Ser232) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: pyruvate dehydrogenase (lipoamide) alpha 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC002406
• Symbole du gène: PDHA1
• Identification du gène (NCBI): 5160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

Publications

Species	Application	Title
mouse	WB	Front Cardiovasc Med; MCC950, a Selective NLRP3 Inhibitor, Attenuates Adverse Cardiac Remodeling Following Heart Failure Through Improving the Cardiometabolic Dysfunction in Obese Mice.; Authors - Menglong Wang
human	WB	FASEB J; PDK4 facilitates fibroblast functions and diabetic wound healing through regulation of HIF-1α protein stability and gene expression; Authors - Zhouji Ma
human,mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron promotes glycolysis to drive colon tumorigenesis; Authors - Zhaoli Liu
mouse	IF	Cell Death Discov; PDK4 rescues high-glucose-induced senescent fibroblasts and promotes diabetic wound healing through enhancing glycolysis and regulating YAP and JNK pathway; Authors - Zhouji Ma