Anticorps Polyclonal de lapin anti-Phospho-P53 (Ser392)
Phospho-P53 (Ser392) Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
WB, ELISA
Conjugaison
Non conjugué
N° de cat : 28963-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | λ phosphatase treated HEK-293 cells, |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 1 publications below |
Informations sur le produit
28963-1-AP cible Phospho-P53 (Ser392) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | Humain |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | tumor protein p53 |
Masse moléculaire calculée | 44 kDa |
Poids moléculaire observé | 53 kDa |
Numéro d’acquisition GenBank | BC003596 |
Symbole du gène | TP53 |
Identification du gène (NCBI) | 7157 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.(PMID: 19935675, PMID: 24379683)
Protocole
Product Specific Protocols | |
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WB protocol for Phospho-P53 (Ser392) antibody 28963-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Chem Biol Interact Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65 |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andria (Verified Customer) (01-29-2023) | This antibody detects 2 bands. I believe the correct one is the lower because it is induced upon actinomycin treatment
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