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Anticorps Monoclonal anti-Phospho-P53 (Ser15)

Phospho-P53 (Ser15) Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

1H6G1

N° de cat : CL555-67826

Synonymes

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite®555-conjugated Phospho-P53 (Ser15) Monocl (CL555-67826)

    FC experiment of HT-29 using CL555-67826

    1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) HT-29 cells were intracellularly stained with 0.13 ug CoraLite®555 Anti-Human Phospho-P53 (Ser15) (CL555-67826, Clone:1H6G1) (red), or 0.13 ug Control Antibody (Blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en cytométrieUV treated HT-29 cells

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-67826 cible Phospho-P53 (Ser15) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet tumor protein p53
Masse moléculaire calculée 44 kDa
Poids moléculaire observé53 kDa
Numéro d’acquisition GenBankBC003596
Symbole du gène TP53
Identification du gène (NCBI) 7157
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)