Anticorps Polyclonal de lapin anti-Phospho-MEK1 (Thr286)

Phospho-MEK1 (Thr286) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB

Conjugaison

Non conjugué

N° de cat : 28933-1-AP

Synonymes

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, Phospho-MEK1 (Thr286), PRKMK1

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Galerie de données de validation

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  WB analysis using 28933-1-AP

  Non-treated HeLa cells and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 28933-1-AP (Phospho-MEK1 (Thr286) antibody) at dilution of 1:3000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: nocodazole treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28933-1-AP cible Phospho-MEK1 (Thr286) dans les applications de WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC139729
• Symbole du gène: MAP2K1
• Identification du gène (NCBI): 5604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 enzymatic activity is regulated by site-specific phosphorylation that can be activated with phosphorylation of Ser217/Ser221 by Raf kinase or suppressed by phosphorylation of Thr286 and Thr292 by CDK1 and CDK5 or Thr292 and Thr386 by ERK1/2. (PMID: 31972311, PMID: 17928366, PMID: 22177953)