Anticorps Recombinant de lapin anti-Phospho-IkB Alpha (Ser32/36)

Phospho-IkB Alpha (Ser32/36) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1G21

N° de cat : 82349-1-RR

Synonymes

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA, Phospho-IkB Alpha (Ser32/36)

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Galerie de données de validation

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  WB analysis of HeLa using 82349-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha-tubulin antibody as loading control.

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  WB analysis using 82349-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82349-1-RR

  PMA treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A, PMA treated HeLa cells

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

82349-1-RR cible Phospho-IkB Alpha (Ser32/36) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004983
• Symbole du gène: NFKBIA
• Identification du gène (NCBI): 4792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha). (PMID: 16904979, PMID: 28990531 PMID: 10455908)

Publications

Species	Application	Title
human	WB	Transl Oncol; Endogenous hydrogen sulfide inhibition suppresses tumor growth by promoting apoptosis and pyroptosis in esophageal cancer cells; Authors - Hong-Gang Wang
rat	WB	Phytomedicine; Ganoderma lucidum polysaccharide peptide (GLPP) attenuates rheumatic arthritis in rats through inactivating NF-κB and MAPK signaling pathways; Authors - Meng Meng
human	WB	Mol Immunol; Catapol attenuates the aseptic inflammatory response to hepatic I/R injury in vivo and in vitro by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway via the microRNA-410-3p; Authors - Zan Jie Feng
human	WB	Am J Physiol Cell Physiol; Inducible nitric oxide synthase activity mediates TNF-α-induced endothelial cell dysfunction; Authors - Chen Liu
human,rat	WB	J Ethnopharmacol; The Terminalia chebula Retz extract treats hyperuricemic nephropathy by inhibiting TLR4/MyD88/NF-κB axis; Authors - Hao Liu
human,mouse	WB	Int J Biol Macromol; In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leaves; Authors - Yang Zhang