Anticorps Recombinant de lapin anti-Phospho-Hamartin/TSC1 (Ser511)

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3N6

N° de cat : CL488-80340

Synonymes

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

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Galerie de données de validation

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  FC experiment of HeLa using CL488-80340

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL488-80340, Clone:3N6), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

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  FC experiment of HeLa using CL488-80340

  1X10^6 HeLa cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.5 ug CoraLite® Plus 488 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL488-80340, Clone:3N6) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

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Applications testées

• Résultats positifs en cytométrie: cellules HeLa traitées à la calyculine A

Dilution recommandée

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-80340 cible Phospho-Hamartin/TSC1 (Ser511) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 1
• Masse moléculaire calculée: 130 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_000368
• Symbole du gène: TSC1
• Identification du gène (NCBI): 7248
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).