Anticorps Polyclonal de lapin anti-Phospho-EIF2S1 (Ser51)

Phospho-EIF2S1 (Ser51) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 28740-1-AP

Synonymes

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51



Applications testées

Résultats positifs en WBcellules PC-3 traitées à la calyculine A,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

28740-1-AP cible Phospho-EIF2S1 (Ser51) dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Masse moléculaire calculée 36 kDa
Poids moléculaire observé36 kDa
Numéro d’acquisition GenBankNM_004094
Symbole du gène EIF2S1
Identification du gène (NCBI) 1965
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51

Protocole

Product Specific Protocols
WB protocol for Phospho-EIF2S1 (Ser51) antibody 28740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation

Authors - Lu Chen
mouse,humanWB,IHC

J Mater Chem B

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating

Authors - Bin Yan
mouseWB

Oxid Med Cell Longev

The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.

Authors - Yichao Du
humanWB

Front Cardiovasc Med

Plasma Purification Treatment Relieves the Damage of Hyperlipidemia to PBMCs.

Authors - Xiao Meng Zhang
mouseWB

J Mol Neurosci

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Authors - Lei Chang
pigWB

Microbiol Spectr

African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway

Authors - Han Zhong