Anticorps Polyclonal de lapin anti-Phospho-CHEK2 (Thr68)

Phospho-CHEK2 (Thr68) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 29012-1-AP

Synonymes

CDS1, CHEK2, CHK2, HuCds1, LFS2, PP1425, RAD53

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Galerie de données de validation

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  WB analysis using 29012-1-AP

  Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29012-1-AP (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:1000 incubated at room temperature for 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules PC-3 traitées au MMS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

29012-1-AP cible Phospho-CHEK2 (Thr68) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: CHK2 checkpoint homolog (S. pombe)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC004207
• Symbole du gène: CHEK2
• Identification du gène (NCBI): 11200
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

Publications

Species	Application	Title
human	WB	Cell Prolif; eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response.; Authors - Chao Mei
human	WB	Front Oncol; ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway; Authors - Xin Wen
human	WB	Research (Wash D C); Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer; Authors - Yangyang Li
human	WB	Cell Death Dis; PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer; Authors - Huan Deng
human	WB	Front Oncol; Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway; Authors - Zhili Xia