Anticorps Polyclonal de lapin anti-Phospho-Beta Catenin (Ser675)

Phospho-Beta Catenin (Ser675) Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 28853-1-AP

Synonymes

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser675), PRO2286, β-Catenin

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Galerie de données de validation

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  WB analysis using 28853-1-AP

  Non-treated HT-29 cells, phosphatase inhibitor and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28853-1-AP (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of MCF-7 using 28853-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (28853-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of MCF-7 using 28853-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (28853-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Applications testées

• Résultats positifs en WB: cellules HT-29 traitées à la λ phosphatase,
• Résultats positifs en IF: λ phosphatase treated MCF-7 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

28853-1-AP cible Phospho-Beta Catenin (Ser675) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: catenin (cadherin-associated protein), beta 1, 88kDa
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC058926
• Symbole du gène: CTNNB1
• Identification du gène (NCBI): 1499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. Beta catenin is a core component of the cadherin protein complex, whose stabilization is essential for the activation of Wnt/β-catenin signaling. Phosphorylation of Ser675 in the C-terminus of at beta catenin promotesmits nuclear accumulation and transcriptional activity, resulting in increased activation of the Wnt/β-catenin signaling pathway(PMID: 25980439). In addition, Ser675 phosphorylation promotes beta catenin interaction with the transcriptional coactivator CREB-binding protein in the nucleus(PMID: 16476742).

Publications

Species	Application	Title
mouse	WB	Endocrinology; Casein kinase 1α regulates testosterone synthesis and testis development in adult mice; Authors - Hongzhou Guo
human	WB	FASEB J; Verteporfin attenuates trauma-induced heterotopic ossification of Achilles tendon by inhibiting osteogenesis and angiogenesis involving YAP/β-catenin signaling; Authors - Gang Luo
human	WB	Bone; Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway; Authors - Yuanxi Leng
human	WB	Cell Rep; LncRNA DGCR5-encoded polypeptide RIP aggravates SONFH by repressing nuclear localization of β-catenin in BMSCs; Authors - Weiqian Jiang
mouse	WB	Ann Transl Med; N-methyl-D-aspartate receptor blockers attenuate bleomycin-induced pulmonary fibrosis by inhibiting endogenous mesenchymal stem cells senescence; Authors - Pu Huang
human	WB	Front Pharmacol; Chebulagic acid suppresses gastric cancer by inhibiting the AURKA/β-catenin/Wnt pathway; Authors - Jing Zhao