Anticorps Polyclonal de lapin anti-Phospho-ALK (Tyr1604)

Phospho-ALK (Tyr1604) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 30266-1-AP

Synonymes

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, Phospho-ALK (Tyr1604), TFG/ALK

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Galerie de données de validation

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  WB analysis using 30266-1-AP

  Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 30266-1-AP (Phospho-ALK (Tyr1604) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as loading control.

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Applications testées

• Résultats positifs en WB: PMA treated HL-60 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30266-1-AP cible Phospho-ALK (Tyr1604) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: anaplastic lymphoma receptor tyrosine kinase
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC157090
• Symbole du gène: ALK
• Identification du gène (NCBI): 238
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The human ALK appears to exist as a 140 kDa protein, as well as the 220 kDa full-length ALK species and the 140 kDa ALK species is thought to be the result of a cleavage within the extracellular region of full-length ALK. The 140 kDa ALK protein is phosphorylated in response to activation of ALK(PMID: 19459784).