Anticorps Monoclonal anti-Periostin

• Phare
• Validé par KD/KO

Periostin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

14

CloneNo.

1A11A3

Avis (1)

N° de cat : 66491-1-Ig

Synonymes

OSF 2, OSF2, Osteoblast specific factor 2, PDLPOSTN, periostin, PN, POSTN, RP11 412K4.1

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Galerie de données de validation

• 

  WB analysis of BxPC-3 using 66491-1-Ig

  BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66491-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66491-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66491-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66491-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66491-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66491-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66491-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66491-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules Neuro-2a, cellules ROS1728, cellules SGC-7901, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2500-1:10000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

66491-1-Ig cible Periostin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Periostin Protéine recombinante Ag14487
• Nom complet: periostin, osteoblast specific factor
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC106710
• Symbole du gène: POSTN
• Identification du gène (NCBI): 10631
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).

Publications

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution; Authors - Taifeng Zhou
human	WB,IHC	Mol Ther; Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration.; Authors - Jinna Wu
human	WB,IHC	Cancer Cell Int; Periostin promotes the proliferation and metastasis of osteosarcoma by increasing cell survival and activates the PI3K/Akt pathway.; Authors - Chaojian Xu ; KD Validated
mouse	WB	Biochem Biophys Res Commun; Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.; Authors - Jie Liu
human	IHC	Aging (Albany NY); An immune signature to predict the prognosis of ATRX-wildtype glioma patients and guide immune checkpoint blockade therapy; Authors - Wenpeng Cao
human	IF,FC	EBioMedicine; Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma; Authors - Shitong Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human fibroblasts