Anticorps Polyclonal de lapin anti-Parvalbumin

Parvalbumin Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 29312-1-AP

Synonymes

D22S749, parvalbumin, Parvalbumin alpha, PVALB

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Galerie de données de validation

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  IHC staining of mouse cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu de cervelet de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cervelet de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

29312-1-AP cible Parvalbumin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Parvalbumin Protéine recombinante Ag30189
• Nom complet: parvalbumin
• Masse moléculaire calculée: 12 kDa
• Numéro d’acquisition GenBank: NM_002854
• Symbole du gène: PVALB
• Identification du gène (NCBI): 5816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation.

Publications

Species	Application	Title
mouse	IF	Cell Rep Methods; A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution; Authors - Jingtan Zhu
mouse	IF	STAR Protoc; Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID; Authors - Jingtan Zhu
mouse	WB	Sci Rep; Identification of mouse soleus muscle proteins altered in response to changes in gravity loading; Authors - Yoko Ino

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Parvalbumin (red) antibody worked in my human brain cortex sections, although it marks mostly the synaptic terminals more than the soma of the parvalbumin cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Parvalbumin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:29312-1-AP)