Anticorps Polyclonal de lapin anti-PYGB

• Phare
• Validé par KD/KO

PYGB Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 55380-1-AP

Synonymes

phosphorylase, glycogen; brain, PYGB

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Galerie de données de validation

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  WB analysis of A549 using 55380-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55380-1-AP

  WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

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  WB analysis of A549 using 55380-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55380-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 55380-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55380-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55380-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 55380-1-AP

  Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules MCF-7, cellules U-251
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

55380-1-AP cible PYGB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phosphorylase, glycogen; brain
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 97-105 kDa
• Numéro d’acquisition GenBank: NM_002862
• Symbole du gène: PYGB
• Identification du gène (NCBI): 5834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.

Publications

Species	Application	Title
mouse	WB	Front Mol Neurosci; Brain-Type Glycogen Phosphorylase Is Crucial for Astrocytic Glycogen Accumulation in Chronic Social Defeat Stress-Induced Depression in Mice.; Authors - Yuanyuan Zhu
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan