Anticorps Polyclonal de lapin anti-PTN

• Phare
• Validé par KD/KO

PTN Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 27117-1-AP

Synonymes

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN

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Galerie de données de validation

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  WB analysis of fetal human brain using 27117-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27117-1-AP (PTN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 27117-1-AP

  U-87 MG (WT and PTN KO) lysates prepared with RIPA buffer, 25μg protein loaded. 55011-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of U-87 MG using 27117-1-AP

  U-87 MG lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- magnetic beads. The Ponceau stained transfers of each blot are shown. SM=2% starting material; UB=2% unbound fraction; IP=immunoprecipitate. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human brain using 27117-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27117-1-AP (PTN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 27117-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27117-1-AP (PTN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules MG U-87
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

27117-1-AP cible PTN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTN Protéine recombinante Ag26004
• Nom complet: pleiotrophin
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC005916
• Symbole du gène: PTN
• Identification du gène (NCBI): 5764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pleiotrophin (PTN) is a multifunctional, cationic, glycosaminoglycan-binding cytokine and growth factor involved in numerous physiological and pathological processes, including tissue repair and inflammation-related diseases. PTN is highly upregulated in the brain in different disorders characterized by overt neuroinflammation such as neurodegenerative diseases, drug addiction, traumatic injury, and ischemia. PTN is important for CNS repair and for survival and differentiation of dopaminergic neurons.

Publications

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao
human	WB	Oncol Rep; Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR‑384/pleiotrophin axis.; Authors - Yuanyuan Yao
human	WB	Oral Dis; PTN inhibited chondrogenic differentiation potential of dental pulp stem cells; Authors - Chang Liu
	IF	Cell Rep; Dissecting the spermatogonial stem cell niche using spatial transcriptomics; Authors - Shreya Rajachandran