Anticorps Monoclonal anti-PTGS1

PTGS1 Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

1F12G7

N° de cat : 67346-1-Ig

Synonymes

COX 1, COX1, COX3, Cyclooxygenase 1, PCOX1, PGG/HS, PGH synthase 1, PGHS 1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, PTGHS, PTGS1

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Galerie de données de validation

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  WB analysis of L02 using 67346-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 67346-1-Ig

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67346-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67346-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67346-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67346-1-Ig (PTGS1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules A431, cellules K-562, leucocyte de sang périphérique humain
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67346-1-Ig cible PTGS1 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTGS1 Protéine recombinante Ag28280
• Nom complet: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 599 aa, 69 kDa
• Poids moléculaire observé: 60-72 kDa
• Numéro d’acquisition GenBank: BC029840
• Symbole du gène: PTGS1
• Identification du gène (NCBI): 5742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.