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Anticorps Monoclonal anti-PTGES3

PTGES3 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C11D11

N° de cat : CL488-67736

Synonymes

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated PTGES3 Monoclonal an (CL488-67736)

    IF Staining of HepG2 using CL488-67736

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PTGES3 antibody (CL488-67736, Clone: 3C11D11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated PTGES3 Monoclonal an (CL488-67736)

    FC experiment of HepG2 using CL488-67736

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTGES3 (CL488-67736, Clone:3C11D11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HepG2,
Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67736 cible PTGES3 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PTGES3 Protéine recombinante Ag7870
Nom complet prostaglandin E synthase 3 (cytosolic)
Masse moléculaire calculée 19 kDa
Poids moléculaire observé22 kDa
Numéro d’acquisition GenBankBC003005
Symbole du gène PTGES3
Identification du gène (NCBI) 10728
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 PTGES3 antibody CL488-67736Download protocol
FC protocol for CL Plus 488 PTGES3 antibody CL488-67736Download protocol
Standard Protocols
Click here to view our Standard Protocols