Anticorps Polyclonal de lapin anti-PTCH2

PTCH2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 55091-1-AP

Synonymes

patched homolog 2 (Drosophila), Protein patched homolog 2, PTC2, PTCH2

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Galerie de données de validation

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  WB analysis of HepG2 using 55091-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55091-1-AP (PTCH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55091-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55091-1-AP (PTCH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

55091-1-AP cible PTCH2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: patched homolog 2 (Drosophila)
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 131-140 kDa
• Numéro d’acquisition GenBank: NM_003738
• Symbole du gène: PTCH2
• Identification du gène (NCBI): 8643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTCH1 (patched homolog 1) and PTCH2 are twelve-pass transmembrane proteins that act receptors of Hedgehog signaling pathway (PMID: 8906794; 9811851). Signals of Hedgehog family proteins (SHH, IHH and DHH in humans) are transduced through Patched family receptors (PTCH1 and PTCH2) and Smoothened (SMO) to GLI family transcription factors. Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). PTCH2 may function as a tumor suppressor in the Hedgehog signaling pathway. Alterations in PTCH2 gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. This antibody is specific to PTCH2.

Publications

Species	Application	Title
rat	WB	Exp Mol Med; Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model.; Authors - Ya-Dong Zhang
human	WB	iScience; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia.; Authors - Xin Chen
mouse	WB	Cell Res; SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches; Authors - Qiuyao Wu