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Anticorps Monoclonal anti-PSME1

PSME1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1F7E2

N° de cat : CL594-67928

Synonymes

IFI5111, IGUP I 5111, PA28A, PA28alpha, PSME1, REG alpha, REGalpha

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated PSME1 Monoclonal antibody (CL594-67928)

    IF Staining of MCF-7 using CL594-67928

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 PSME1 antibody (CL594-67928, Clone: 1F7E2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated PSME1 Monoclonal antibody (CL594-67928)

    FC experiment of MCF-7 using CL594-67928

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSME1 (CL594-67928, Clone:1F7E2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules MCF-7,
Résultats positifs en cytométriecellules MCF-7

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67928 cible PSME1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PSME1 Protéine recombinante Ag30549
Nom complet proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Masse moléculaire calculée 29 kDa
Poids moléculaire observé29 kDa
Numéro d’acquisition GenBankBC007503
Symbole du gène PSME1
Identification du gène (NCBI) 5720
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Protocole

Product Specific Protocols
IF protocol for CL594 PSME1 antibody CL594-67928Download protocol
FC protocol for CL594 PSME1 antibody CL594-67928Download protocol
Standard Protocols
Click here to view our Standard Protocols