Anticorps Polyclonal de lapin anti-PSMB11

PSMB11 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 55143-1-AP

Synonymes

beta5t, Proteasome subunit beta 5t, PSMB11

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Galerie de données de validation

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  WB analysis of Jurkat using 55143-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55143-1-AP (PSMB11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 55143-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thymus using 55143-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 55143-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PSMB11 (55143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en IHC: tissu de thymus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:600
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55143-1-AP cible PSMB11 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 11
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: NM_001099780
• Symbole du gène: PSMB11
• Identification du gène (NCBI): 122706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anja (Verified Customer) (03-16-2022)

We tested the antibody in different dilutions on different cells of the thymus and Psmb11 knockout (KO) mice and could not obtain a specific staining with the antibody. In our hands the signal in the Psmb11-KO mice was comparable to the one obtained from either thymocytes or thymus epithelial cells of Wildtype mice .

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Thymus