Anticorps Recombinant de lapin anti-PSD95-Specific,DLG4

PSD95-Specific,DLG4 Recombinant Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1I22

Avis (1)

N° de cat : 81106-1-RR

Synonymes

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

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Galerie de données de validation

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  WB analysis using 81106-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 81106-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 81106-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 81106-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81106-1-RR cible PSD95-Specific,DLG4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: discs, large homolog 4 (Drosophila)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: NM_001365
• Symbole du gène: DLG4
• Identification du gène (NCBI): 1742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Baptiste (Verified Customer) (05-19-2023)

Good results at 1/1000 dilution on mouse brain slices. This was our first use of a recombinant antibody and it is entirely comparable to a traditional one.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse brain slice