Anticorps Polyclonal de lapin anti-PRPS2

• Phare
• Validé par KD/KO

PRPS2 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 27024-1-AP

Synonymes

PPRibP, PRPS2, PRS II, PRSII

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Galerie de données de validation

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  WB analysis of A375 using 27024-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27024-1-AP (PRPS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 27024-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27024-1-AP (PRPS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 27024-1-AP

  IP result of anti-PRPS2(IP:27024-1-AP, 4ug; Detection:27024-1-AP 1:1000) with mouse spleen tissue lysate 1560 ug.

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  IP experiment of A375 using 27024-1-AP

  IP result of anti-PRPS2(IP:27024-1-AP, 4ug; Detection:27024-1-AP 1:1000) with A375 cells lysate 1600 ug.

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Applications testées

• Résultats positifs en WB: cellules A375
• Résultats positifs en IP: cellules A375, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

27024-1-AP cible PRPS2 dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPS2 Protéine recombinante Ag25785
• Nom complet: phosphoribosyl pyrophosphate synthetase 2
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC119662
• Symbole du gène: PRPS2
• Identification du gène (NCBI): 5634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRPS (phosphoribosyl pyrophosphate synthetase) proteins catalyze the synthesis of phosphoribosyl pyrophosphate (PRPP). Three human PRPS isoforms exist and are encoded by three different genes. PRPS1 and PRPS2 (also known as PRS1 and PRS2, respectively) are ubiquitously expressed, while PRPS3 (also known as PRPS1L1) is specific to the testis. PRPP is an important substrate synthesized from MgATP and ribose-5-phosphate in a reaction that requires inorganic phosphate and magnesium as a cofactor. PRPP is essential in the synthesis of nearly all nucleotides, implying that PRPS proteins play an important role in nucleotide biosynthesis and purine metabolism. PRPS2 is a 318 amino acid protein that exists as a homodimer and a hexamer composed of three homodimers.

Publications

Species	Application	Title
human	CoIP,WB	Sci Adv; NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.; Authors - Shashank Srivastava
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv ; KD Validated
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran
human	WB	Nat Commun; Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress; Authors - Ronghui Yang ; KD Validated