Anticorps Monoclonal anti-PRKCB

PRKCB Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

4B7A1

N° de cat : 68620-1-Ig

Synonymes

PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta

---

Galerie de données de validation

• 

  WB analysis using 68620-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68620-1-Ig (PRKCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

68620-1-Ig cible PRKCB dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRKCB Protéine recombinante Ag33693
• Nom complet: protein kinase C, beta
• Masse moléculaire calculée: 673 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC036472
• Symbole du gène: PRKCB
• Identification du gène (NCBI): 5579
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Publications

Species	Application	Title
	WB	Redox Biol; Empagliflozin prevents oxidative stress in human coronary artery endothelial cells via the NHE/PKC/NOX axis; Authors - Xiaoling Li