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Anticorps Monoclonal anti-PRKAR2A

PRKAR2A Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2B3

N° de cat : CL488-67751

Synonymes

PKR2, PRKAR2, PRKAR2A

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PRKAR2A Monoclonal a (CL488-67751)

    IF Staining of HeLa using CL488-67751

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKAR2A antibody (CL488-67751, Clone: 1B2B3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated PRKAR2A Monoclonal a (CL488-67751)

    FC experiment of MCF-7 using CL488-67751

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRKAR2A (CL488-67751, Clone:1B2B3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules MCF-7

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67751 cible PRKAR2A dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PRKAR2A Protéine recombinante Ag29893
Nom complet protein kinase, cAMP-dependent, regulatory, type II, alpha
Masse moléculaire calculée 43 kDa
Poids moléculaire observé50 kDa
Numéro d’acquisition GenBankBC002763
Symbole du gène PRKAR2A
Identification du gène (NCBI) 5576
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 PRKAR2A antibody CL488-67751Download protocol
FC protocol for CL Plus 488 PRKAR2A antibody CL488-67751Download protocol
Standard Protocols
Click here to view our Standard Protocols