Anticorps Polyclonal de lapin anti-PRKACB

PRKACB Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 55382-1-AP

Synonymes

PKA C beta, PKA C-beta-specific, PKACB, PRKACB

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Galerie de données de validation

• 

  WB analysis of mouse brain using 55382-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55382-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55382-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55382-1-AP (PKA C-beta-specifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55382-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 55382-1-AP (PKA C-beta-specifc antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 55382-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 55382-1-AP (PKA C-beta-specifc antibody at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 3 publications below

Informations sur le produit

55382-1-AP cible PRKACB dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, cAMP-dependent, catalytic, beta
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 36-55 kDa
• Numéro d’acquisition GenBank: NM_002731
• Symbole du gène: PRKACB
• Identification du gène (NCBI): 5567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. This antibody is specific to PRKACB.

Publications

Species	Application	Title
human,mouse	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
mouse	WB	Int J Mol Sci; Per1/Per2 Disruption Reduces Testosterone Synthesis and Impairs Fertility in Elderly Male Mice.; Authors - Qinrui Liu
human	WB	Oncogenesis; GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.; Authors - Chenggang Zhao
human	WB	Pharmacol Res; Hypericin attenuates nonalcoholic fatty liver disease and abnormal lipid metabolism via the PKA-mediated AMPK signaling pathway in vitro and in vivo.; Authors - Chen Liang
human	IHC	Am J Cancer Res; Different lipid metabolic profiles and their associated genes in sessile serrated adenoma or polyps compared to hyperplastic polyps.; Authors - Chaotao Tang
human	WB	Viruses; ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells; Authors - Beibei Zhang