Anticorps Polyclonal de lapin anti-PKA C-alpha specific

PKA C-alpha specific Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 55388-1-AP

Synonymes

PKA C alpha, PKACA, PRKACA

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Galerie de données de validation

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  WB analysis of human testis using 55388-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 55388-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 55388-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 55388-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55388-1-AP

  IP Result of anti-PKA C-alpha speicifc (IP:55388-1-AP, 4ug; Detection:55388-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human colon cancer using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human colon cancer using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human testis using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human testis using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu hépatique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55388-1-AP cible PKA C-alpha specific dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, cAMP-dependent, catalytic, alpha
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 38-43 kDa
• Numéro d’acquisition GenBank: NM_002730
• Symbole du gène: PRKACA
• Identification du gène (NCBI): 5566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.

Publications

Species	Application	Title
human	WB,IF,IP	Sci Total Environ; Prolonged exposure of environmental concentration benzo[a]pyrene promoted cancer stemness through AhR/PKA/SOX2 dependent pathway in small cell lung cancer; Authors - Heng Ni
		Oncotarget; Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland.; Authors - Laura C Hernández-Ramírez
mouse	WB	Front Pharmacol; An Integrative Pharmacology-Based Strategy to Uncover the Mechanism of Xiong-Pi-Fang in Treating Coronary Heart Disease with Depression.; Authors - Lihong Zhang
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
human	WB	Proteomics; Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein Upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.; Authors - Hwee Tong Tan
		CNS Neurosci Ther; Increased Expression of Transient Receptor Potential Vanilloid 4 in Cortical Lesions of Patients with Focal Cortical Dysplasia.; Authors - Xin Chen