Anticorps Polyclonal de lapin anti-PRDM15

• Phare
• Validé par KD/KO

PRDM15 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 25590-1-AP

Synonymes

C21orf83, PFM15, PR domain containing 15, PRDM15, Zinc finger protein 298, ZNF298

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Galerie de données de validation

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  WB analysis of Jurkat cells using 25590-1-AP

  WB result of PRDM15 (25590-1-AP, 1:500) with si-control and si-PRDM15 transfected Jurkat cells.

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  WB analysis of MCF-7 using 25590-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25590-1-AP (PRDM15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 25590-1-AP

  IP Result of anti-PRDM15 (IP:25590-1-AP, 5ug; Detection:25590-1-AP 1:500) with MCF-7 cells lysate 2500ug.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules Jurkat
• Résultats positifs en IP: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

25590-1-AP cible PRDM15 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRDM15 Protéine recombinante Ag22296
• Nom complet: PR domain containing 15
• Masse moléculaire calculée: 1507 aa, 169 kDa
• Poids moléculaire observé: 169 kDa
• Numéro d’acquisition GenBank: BC067102
• Symbole du gène: PRDM15
• Identification du gène (NCBI): 63977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PR-domain containing proteins (PRDMs) have a common involvement in the modulation of gene activities. A PR-domain family member usually produces two products, called PR-plus and PR-minus, which differ by the presence or absence of the PR domain, respectively. The PR-plus product is underexpressed or disrupted in cancer cells, whereas the PR-minus product is present or overexpressed in cancer cells. This imbalance in the amount of the two products, which is a result of either genetic or epigenetic events, appears to be a determining factor of malignancy. PRDM15 (PR domain-containing protein 15), also known as PFM15 or ZNF298, is a 1507 amino acid protein belonging to the PRDM family. Localizing to the nucleus, PRDM15 contains sixteen C2H2-type zinc fingers and one SET domain. It is believed to participate in transcriptional regulation and may be involved in cell differentiation and tumorigenesis. PRDM15 exists several isoform and molecular weight of respective isoform is 169 kDa, 135kda, 62kDa.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; The RNA methyltransferase METTL16 enhances cholangiocarcinoma growth through PRDM15-mediated FGFR4 expression; Authors - Nianli Liu ; KD Validated