Anticorps Monoclonal anti-PPP3CB

PPP3CB Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3C1A3

N° de cat : 66614-1-Ig

Synonymes

CALNA2, CALNB, CAM PRP catalytic subunit, CNA2, PPP3CB

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Galerie de données de validation

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  WB analysis using 66614-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66614-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66614-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66614-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66614-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

66614-1-Ig cible PPP3CB dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP3CB Protéine recombinante Ag26987
• Nom complet: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Masse moléculaire calculée: 525 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC028049
• Symbole du gène: PPP3CB
• Identification du gène (NCBI): 5532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin.

Publications

Species	Application	Title
human,mouse	WB	Autophagy; Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin; Authors - Zhiyuan Fang ; KD Validated