Anticorps Polyclonal de lapin anti-Neurabin 2

• Phare
• Validé par KD/KO

Neurabin 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 55129-1-AP

Synonymes

Neurabin 2, Neurabin II, PPP1R6, PPP1R9, PPP1R9B, SPINO, Spinophilin, Spn

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Galerie de données de validation

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  WB analysis of mouse brain using 55129-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55129-1-AP (Neurabin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55129-1-AP

  WB result of PPP1R9B antibody (55129-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neurabin 2 transfected A549 cells.

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  IP experiment of mouse brain using 55129-1-AP

  IP Result of anti-Neurabin 2 (IP:55129-1-AP, 4ug; Detection:55129-1-AP 1:300) with mouse brain tissue lysate 6000ug.

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  IHC staining of human brain using 55129-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55129-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

55129-1-AP cible Neurabin 2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_032595
• Symbole du gène: PPP1R9B
• Identification du gène (NCBI): 84687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).

Publications

Species	Application	Title
human	WB,IP	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
	WB	Toxicology; Changes in memory and synaptic plasticity induced in male rats after maternal exposure to Bisphenol A.; Authors - Chong Wang
mouse	WB	Genes Dis; Hippocampal overexpression of TREM2 ameliorates high fat diet induced cognitive impairment and modulates phenotypic polarization of the microglia.; Authors - Min Wu