Anticorps Monoclonal anti-PPAR Gamma

• Phare
• Validé par KD/KO

PPAR Gamma Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

4E12F10

N° de cat : 60127-1-Ig

Synonymes

CIMT1, NR1C3, PPAR gamma, PPARG1

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Galerie de données de validation

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  WB analysis using 60127-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 60127-1-Ig

  WB result of PPAR Gamma antibody (60127-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAR Gamma transfected HL-60 cells.

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  IHC staining of human prostate cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 60127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon using 60127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of K-562 using 60127-1-Ig

  1X10^6 K-562 cells were stained with 0.20ug PPAR gamma antibody (60127-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules THP-1
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de côlon de rat, tissu de côlon de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de côlon humain,
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

60127-1-Ig cible PPAR Gamma dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPAR Gamma Protéine recombinante Ag10005
• Nom complet: peroxisome proliferator-activated receptor gamma
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC006811
• Symbole du gène: PPARG
• Identification du gène (NCBI): 5468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publications

Species	Application	Title
mouse	WB	J Physiol Biochem; miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.; Authors - Chen Chen
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
mouse	IHC	J Immunol Res; Identification of Adipogenesis Subgroups and Immune Infiltration Characteristics in Diabetic Peripheral Neuropathy; Authors - Yumin Lin
sheep	WB	Int J Mol Sci; The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432; Authors - Meilin Jin