Anticorps Monoclonal anti-POLR3D

• Phare
• Validé par KD/KO

POLR3D Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2F5G4

N° de cat : 68263-1-Ig

Synonymes

BN51, BN51T, POLR3D, Protein BN51, RNA polymerase III subunit C4, RPC4, RPC53, RPC53 homolog, TSBN51

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Galerie de données de validation

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  WB analysis of HEK-293 using 68263-1-Ig

  WB result of POLR3D antibody (68263-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR3D transfected HEK-293 cells.

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  WB analysis using 68263-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rabbit brain using 68263-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 68263-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, tissu cérébral de lapin

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68263-1-Ig cible POLR3D dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin

• Réactivité: Humain, Lapin
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: POLR3D Protéine recombinante Ag7646
• Nom complet: polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC004484
• Symbole du gène: POLR3D
• Identification du gène (NCBI): 661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.