Anticorps Polyclonal de lapin anti-PMS1

PMS1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 28186-1-AP

Synonymes

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

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Galerie de données de validation

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  WB analysis using 28186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28186-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 28186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (28186-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules K-562, cellules MOLT-4
• Résultats positifs en IF: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28186-1-AP cible PMS1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PMS1 Protéine recombinante Ag28132
• Nom complet: PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
• Poids moléculaire observé: 106 kDa
• Numéro d’acquisition GenBank: BC096331
• Symbole du gène: PMS1
• Identification du gène (NCBI): 5378
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniela (Verified Customer) (03-17-2022)

The staining was aspecific and very weak.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Gastric mouse organoids

PMS1 Antibody Immunofluorescence validation (1:100 dilution) in Gastric mouse organoids (Cat no:28186-1-AP)