Anticorps Polyclonal de lapin anti-PLS1

• Phare
• Validé par KD/KO

PLS1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 55212-1-AP

Synonymes

I plastin, Intestine specific plastin, Plastin 1, plastin 1 (I isoform), PLS1

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Galerie de données de validation

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  WB analysis of mouse small intestine using 55212-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 55212-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 55212-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris, cellules COLO 320
• Résultats positifs en IHC: tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

55212-1-AP cible PLS1 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: plastin 1 (I isoform)
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_002670
• Symbole du gène: PLS1
• Identification du gène (NCBI): 5357
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.

Publications

Species	Application	Title
human	WB,IHC	Cancer Sci; Plastin 1 drives metastasis of colorectal cancer through the IQGAP1/Rac1/ERK pathway.; Authors - Tongtong Zhang ; KD Validated