Anticorps Polyclonal de lapin anti-Perilipin 1

Perilipin 1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27716-1-AP

Synonymes

PERI, perilipin, Perilipin 1, PLIN, PLIN1

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Galerie de données de validation

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (Perilipin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (PLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules 3T3-L1, cellules MCF-7, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IF: tissu adipeux brun de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

27716-1-AP cible Perilipin 1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Perilipin 1 Protéine recombinante Ag26426
• Nom complet: perilipin
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 62-67 kDa
• Numéro d’acquisition GenBank: NM_001145311
• Symbole du gène: PLIN
• Identification du gène (NCBI): 5346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and is a modulator of adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that derives in an abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Publications

Species	Application	Title
mouse	WB	Hum Gene Ther; Kallistatin Improves High-Fat-Induced Insulin Resistance via Epididymal Adipose Tissue-Derived Exosomes; Authors - Zi-Wei Yang
human,mouse	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du