Anticorps Polyclonal de lapin anti-PLEKHA1

PLEKHA1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 26830-1-AP

Synonymes

PLEKHA1, TAPP 1, TAPP1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 26830-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26830-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26830-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 26830-1-AP

  IP result of anti-PLEKHA1 (IP:26830-1-AP, 4ug; Detection:26830-1-AP 1:1000) with A549 cells lysate 1600 ug.

• 

  IHC staining of human colon using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 26830-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, cellules MOLT-4
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26830-1-AP cible PLEKHA1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLEKHA1 Protéine recombinante Ag25285
• Nom complet: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC001136
• Symbole du gène: PLEKHA1
• Identification du gène (NCBI): 59338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."