Anticorps Polyclonal de lapin anti-PKMYT1

PKMYT1 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 55098-1-AP

Synonymes

MYT1, Myt1 kinase, PKMYT1

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Galerie de données de validation

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  WB analysis of mouse brain using 55098-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 55098-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55098-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 55098-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 55098-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A375, cellules HeLa, cellules HepG2, cellules HL-60, tissu cutané de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

55098-1-AP cible PKMYT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, membrane associated tyrosine/threonine 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: NM_004203
• Symbole du gène: PKMYT1
• Identification du gène (NCBI): 9088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

Publications

Species	Application	Title
human	WB	Thorac Cancer; Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3; Authors - Dong Zhou