Anticorps Polyclonal de lapin anti-PKC Zeta

PKC Zeta Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 26899-1-AP

Synonymes

PKC ZETA, PKC2, PRKCZ, Protein kinase C zeta type, protein kinase C, zeta



Applications testées

Résultats positifs en WBcellules HEK-293T, cellules HT-29
Résultats positifs en IFcellules HeLa, cellules NIH/3T3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 4 publications below

Informations sur le produit

26899-1-AP cible PKC Zeta dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PKC Zeta Protéine recombinante Ag25391
Nom complet protein kinase C, zeta
Masse moléculaire calculée 80 kDa
Poids moléculaire observé 67 kDa
Numéro d’acquisition GenBankBC014270
Symbole du gène PRKCZ
Identification du gène (NCBI) 5590
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.

Protocole

Product Specific Protocols
WB protocol for PKC Zeta antibody 26899-1-APDownload protocol
IF protocol for PKC Zeta antibody 26899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

HRS plays an important role for TLR7 signaling to orchestrate inflammation and innate immunity upon EV71 infection.

Authors - Zhen Luo
mouseWB

JCI Insight

Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.

Authors - Hongyi Zhou
WB

Fish Shellfish Immunol

In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.

Authors - Zhong-Wen Chang
mouseWB

J Cell Mol Med

Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis.

Authors - Qianxia Yin