Anticorps Monoclonal anti-PHLPP

PHLPP Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2E8E6

N° de cat : 67640-1-Ig

Synonymes

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

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Galerie de données de validation

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  WB analysis of mouse brain using 67640-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67640-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67640-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67640-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de porc, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

67640-1-Ig cible PHLPP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PHLPP Protéine recombinante Ag18982
• Nom complet: PH domain and leucine rich repeat protein phosphatase
• Masse moléculaire calculée: 1717 aa, 184 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC126277
• Symbole du gène: PHLPP
• Identification du gène (NCBI): 23239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway.; Authors - Zong-Ze Zhang
pig	WB	Pediatr Res; Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets; Authors - Agus Suryawan
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu