Anticorps Polyclonal de lapin anti-PGAP1

PGAP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 55392-1-AP

Synonymes

Bst1, GPI inositol deacylase, hPGAP1, ISPD3024, PGAP1

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Galerie de données de validation

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  WB analysis of human testis using 55392-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55392-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55392-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 55392-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55392-1-AP (PGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55392-1-AP (PGAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55392-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PGAP1 antibody (55392-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules HEK-293, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

55392-1-AP cible PGAP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: post-GPI attachment to proteins 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 49-53 kDa, 85-90 kDa
• Numéro d’acquisition GenBank: NM_024989
• Symbole du gène: PGAP1
• Identification du gène (NCBI): 80055
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1.

Publications

Species	Application	Title
human	WB	Mol Cell; Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells; Authors - Tatsuaki Kurosaki
human	WB	J Biol Chem; Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.; Authors - Takahiro Nakane

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

pitna (Verified Customer) (12-19-2019)

PGAP1According to Uniprot, this protein has 4 isoforms ranging from 49 – 105 KDa. it appears that the regulation of the different isoforms is still an open question in the literatureTo reduce non-specific binding, blots were incubated in Odyssey Blocking buffer (Li-Cor) for 1 hour at room temperature (RT) .

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)

PGAP1 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:55392-1-AP)