Anticorps Polyclonal de lapin anti-PGAM5

PGAM5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 28445-1-AP

Synonymes

BXLBv68, PGAM5

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Galerie de données de validation

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  WB analysis using 28445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28445-1-AP (PGAM5 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 28445-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PGAM5 antibody (28445-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

28445-1-AP cible PGAM5 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGAM5 Protéine recombinante Ag28195
• Nom complet: phosphoglycerate mutase family member 5
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC008196
• Symbole du gène: PGAM5
• Identification du gène (NCBI): 192111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

Publications

Species	Application	Title
human,mouse	IHC,IF	Cell Biosci; Differential effects of PGAM5 knockout on high fat high fructose diet and methionine choline-deficient diet induced non-alcoholic steatohepatitis (NASH) in mice; Authors - Li Li
human,mouse	WB	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	WB,IF	Int Immunopharmacol; 4-Octyl itaconate inhibits inflammation to attenuate psoriasis as an agonist of oxeiptosis; Authors - Mengshu You
human,pig	WB,CoIP	J Virol; PGAM5 degrades PDCoV N protein and activates type I interferon to antagonize viral replication; Authors - Xinyu Yang
mouse	WB	Biochim Biophys Acta Mol Cell Res; Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer's Disease.; Authors - Marcel V Alavi
human	WB	Acta Biochim Biophys Sin (Shanghai); PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation; Authors - Gongyu Fu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (07-03-2020)

1:1000 dilution in 1%BSA giving a very clear results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human