Anticorps Monoclonal anti-PELP1

PELP1 Monoclonal Antibody for FC (Intra), IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B11E4

Avis (1)

N° de cat : 67050-1-Ig

Synonymes

HMX3, MNAR, P160, PELP1, Transcription factor HMX3

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Galerie de données de validation

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  WB analysis using 67050-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 67050-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67050-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 67050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of MCF-7 using 67050-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MCF-7, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

67050-1-Ig cible PELP1 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PELP1 Protéine recombinante Ag25729
• Nom complet: proline, glutamate and leucine rich protein 1
• Masse moléculaire calculée: 1130 aa, 120 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC069058
• Symbole du gène: PELP1
• Identification du gène (NCBI): 27043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.

Publications

Species	Application	Title
mouse	WB,IHC	Int Immunopharmacol; MiR-195-5p is involved in testicular ischemia/reperfusion injury by directly targeting PELP1 and regulating spermatogonia pyroptosis; Authors - Kai-Xiang He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

fatemeh (Verified Customer) (11-30-2021)

The antibody works well on human protein lystae (30 ug of protein from Hek293 cells, 8% gel, wet transfer 90min 300mA in tris-Gly buffer on PVDF, incubation with primary ab was onvernight at 4C). we coulnt see the specific band for mouse cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1-500
• Cell Tissue Type: Hek293 (human), E14 (mouse)

PELP1 Antibody Western Blot validation (1-500 dilution) in Hek293 (human), E14 (mouse) (Cat no:67050-1-Ig)