Anticorps Monoclonal anti-PEDF

PEDF Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

2C5C8

N° de cat : 66554-1-Ig

Synonymes

EPC 1, PEDF, PIG35, Serpin F1, SERPINF1

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Galerie de données de validation

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  WB analysis of human plasma using 66554-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig plasma using 66554-1-Ig

  pig plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 66554-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, tissu plasmatique de porc, tissu sanguin humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66554-1-Ig cible PEDF dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PEDF Protéine recombinante Ag26660
• Nom complet: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Masse moléculaire calculée: 418 aa, 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC013984
• Symbole du gène: SERPINF1
• Identification du gène (NCBI): 5176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.