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Anticorps Monoclonal anti-PEBP1

PEBP1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4D11

N° de cat : CL488-66438

Synonymes

HCNP, HCNPpp, Neuropolypeptide h3, PBP, PEBP, PEBP 1, PEBP1, Prostatic binding protein, Raf kinase inhibitor protein, RKIP

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated PEBP1 Monoclonal ant (CL488-66438)

    IF Staining of A549 using CL488-66438

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using CoraLite® Plus 488 PEBP1 antibody (CL488-66438, Clone: 2D4D11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated PEBP1 Monoclonal ant (CL488-66438)

    FC experiment of PC-12 using CL488-66438

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PEBP1 (CL488-66438, Clone:2D4D11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules A549,
Résultats positifs en cytométriecellules PC-12

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66438 cible PEBP1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PEBP1 Protéine recombinante Ag0864
Nom complet phosphatidylethanolamine binding protein 1
Masse moléculaire calculée 21 kDa
Poids moléculaire observé 23-27 kDa
Numéro d’acquisition GenBankBC008714
Symbole du gène PEBP1
Identification du gène (NCBI) 5037
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 PEBP1 antibody CL488-66438Download protocol
FC protocol for CL Plus 488 PEBP1 antibody CL488-66438Download protocol
Standard Protocols
Click here to view our Standard Protocols